[ 생화학 ]
완충용액제조
및 아미노산적정
pH와 완충용액 I Korea Univ.
pH와 완충용액 II Yonsei Univ.
아미노산과 단백질의 정성, 정량분석 Protein 특성(Bradford assay) Korea Univ.
Methods for Protein concentration Determination Yonsei Univ.
아미노산의 분리 및 정성분석 Yonsei Univ.
단백질의
과발현과
크로마토그래피
Cell and Protein Harvest KAIST
Ion Exchange Chromatography KAIST
Protein Induction by IPTG KAIST
MBP-tag Affinity Chromatography KAIST
Affinity chromatography를 이용한 단백질 정제 Seoul Nat`l Univ.
Protein Overexpression in E. coli and Purification by Affinity Chromatography Yonsei Univ.
Purification of Thermostable DNA polymerase Yonsei Univ.
겔 거르기 크로마토그래피I Yonsei Univ.
효소 활성과
반응속도론
Enzyme Activity Test KAIST
흡광광도법 Yonsei Univ.
효소반응속도론 Yonsei Univ.
지방질 추출
및 정성분석
동물 및 식물 조직으로부터의 지질추출 및 정성분석 Yonsei Univ.
탄수화물분리
및 정량
간 조직으로부터의 탄수화물 분리 및 정성분석 Yonsei Univ.
SDS-PAGE와
Western blot
Coomassie Staining KAIST
SDS-PAGE KAIST
SDS-PAGE Korea Univ.
SDS gel electrophoresis Seoul Nat`l Univ.
SDS-PAGE & Bradford Assay Yonsei Univ.
Western Blot Analysis using His-Tag Antibody Yonsei Univ.
세포 내 단백질 추출물 분리 및 분석 물리, 화학적 성질을 이용한 세포 추출물 분리 POSTECH
Reporter gene assay/BCA assay Seoul Nat`l Univ.
peptide finger printing Peptide Mass Fingerprinting I Yonsei Univ.
Peptide Mass Fingerprinting II Yonsei Univ.
 
[ 분자생물학 ]
Plasmid DNA 조작과 transformation DNA Gel Electrophoresis KAIST
Preparation of Recombinant DNA Korea Univ.
Recombinant DNA Transformation Korea Univ.
Vector DNA Mini-prep Korea Univ.
Vector Transformation Seoul Nat`l Univ.
Making competent cell Seoul Nat`l Univ.
Plasmid prep using CsCl2 Seoul Nat`l Univ.
Gene cloning II (Restriction analysis & Ligation) Yonsei Univ.
Gene cloning III (transformation) Yonsei Univ.
DNA preparation Yonsei Univ.
Isolation of plasmid DNA from E.coli Yonsei Univ.
Restriction enzyme/Agarose gel electrophoresis POSTECH
세균배양 Seoul Nat`l Univ.
Midi-Scale DNA Preparation Yonsei Univ.
Sub-Cloning (I): Restriction digestion, elution and ligation of DNA Yonsei Univ.
Sub-Cloning (II): Restriction mapping of circular plasmid, Transformation of ligated DNA Yonsei Univ.
Sub-Cloning (III): Detection of subcloning Yonsei Univ.
세포 분리와 분석 FACS를 이용한 세포 주기 관찰 POSTECH
Cytotoxicity text (MTT assay) Seoul Nat`l Univ.
생쥐 비장 면역세포 분리, 염색 및 유세포 분석 Yonsei Univ.
원심분리를 이용한 마우스 혈액 면역세포 분리 Yonsei Univ.
진핵세포
transfection
Mammalian cell transfection with calcium POSTECH
Mammalian cell transfection with liposome POSTECH
Protoplast transformation POSTECH
Mammalian cell culture Seoul Nat`l Univ.
Transfection on mammalian cells Seoul Nat`l Univ.
역전사와 PCR반응 PCR of Insert (T4 Ligase) Gene KAIST
real time qPCR POSTECH
Gene cloning I (PCR) Korea Univ.
Polymerase Chain Reaction/Agarose gel electrophoresis Seoul Nat`l Univ.
PCR-Mediated Mutagenesis (for Two Weeks) Yonsei Univ.
RT-PCR Analysis Yonsei Univ.
진핵세포의 RNA와
genomic DNA분리
Genomic DNA from monolayer cell POSTECH
Genomic DNA from mouse tails POSTECH
Genomic DNA from plant POSTECH
RNA prep from plant POSTECH
RNA isolation from monolayer cells by using Trizol reagent Yonsei Univ.